Immobilized Enzyme Reactors Based on Nucleoside Phosphorylases (NPs) and Nucleoside 2′-Deoxyribosyltransferases (NDTs) for the In-Flow Synthesis of Nucleoside Analogues

Thermus thermophilus nucleoside phosphorylases active in the synthesis of nucleoside analogues.

Cells extracts from Thermus thermophilus HB27 express phosphorolytic activities on purines and pyrimidine nucleosides. Five putative encoding genes were cloned and expressed in Escherichia coli, and the corresponding recombinant proteins were purified and studied. Two of these showed phosphorolytic activities against purine nucleosides, and third one showed phosphorolytic activity against pyrim...

Chemical Synthesis of Nucleoside Analogues Chemical Synthesis of Nucleoside Analogues

Convenient synthesis of nucleoside and isonucleoside analogues.

[reaction: see text]. A very simple methodology to stereoselectively achieve tricyclic isonucleosides (nucleobase = thymine, uracil, and 5-fluoruracil) and 3'-C-branched nucleosides (nucleobase = theophylline) was performed by means of a DBU-mediated addition process using a readily available 2-bromo sugar. The mechanism for these transformations implies the loss of both substituents at C-2 and...

Synthesis of 1,2,3- Triazole Nucleoside Analogues

Both 1,2,3-triazoles and nucleosides have proved to be useful pharmacophores for antiviral and anticancer agents. A series of novel 1,2,3-triazole nucleoside analogues were designed and synthesized in order to find new antiviral or antitumor compounds. KEYWORD: Triazole; Nucleoside; Virus; Cancer International Conference on Industrial Technology and Management Science (ITMS 2015) © 2015. The au...

Nucleoside Analogues: Synthesis from Strained Rings

Nucleoside analogues are widely employed as bioactive compounds against cancer and viral infections. Consequently, it is important to develop efficient synthetic methods to access them with high efficiency and structural diversity. Herein, we present a full account of our work on the synthesis of nucleoside analogues via annulations of donor acceptor aminocyclopropanes and aminocyclobutanes. Th...